An introduction to one- and two-dimensional lineshape analysis of chemically exchanging systems

Molecules are dynamic entities, and understanding intra- inter-molecular reactions changes in conformation is one of the most fascinating, important complex subjects NMR. Conformational chemical result observed spins exchanging between different magnetic environments, sensitivity NMR spectra to such processes has been recognised since earliest days field. Careful analysis spectra, acquired using one- or two-dimensional experiments, can provide insight into structural, thermodynamic, kinetic mechanistic aspects underlying exchange process. The theoretical principles these lineshape methods will be introduced this article, alongside a practical discussion calculation methods, data acquisition software.